Click Run. 3. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seProducts : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. The readings of samples incubated with different carbon sources were subtracted from the average of the control readings. Plaque reduction. • DNA/RNA quantitation • Reporter gene assay The FilterMax™ F5 features absorbance (UV and visible), fluorescence intensity - FI (top and bottom reading), glow luminescence, fluorescence polarization (FP), and time-resolved fluorescence (TRF) for 6- to 1536-well plates. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your intial investment. FilterMax™ F3 and F5 Multimode Microplate Readers, Molecular Devices. 40266 Email: drugdiscoverytech@lifetech. All configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking and comprehensive data management using our SoftMax® Pro Microplate DataFilterMax ™ F5 Multi-Mode Microplate Reader; SpectraMax L Microplate Luminometer; Methods. The experiment was carried out with four biological replicates and two technical replicates. Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm), Emission Filter Slide #1 (Fluorescence: 535. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumAfter incubation for 6 minutes, the mixture was read at 340 nm using a microplate reader (FilterMax F3/F5 Multi-Mode Microplate Readers, Molecular Devices). Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm), Emission Filter Slide #1 (Fluorescence: 535. , a long-time leader in bioanalytical systems for microplate analysis, today announced the launch of two new multi-mode detection platforms that expand its portfolio of microplate readers, enabling the company to meet all combinations of customer needs now and into the future. Labsystems Fluoroskan Ascent FL Microplate Fluorometer $3,250. 00The microplate reader market was valued at US$ 534. Luminescence (glow) Fluorescence polarization. 8. Products : General, FilterMax F5 reader. After further centrifugation in the same fashion, the supernatant was subjected to subsequent enzymatic reactions. Trinean Dropsense 96 Polychromatic. Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The ELISA test was performed using the supernatants from the morphological test. Enzymatic activity was calculated after subtracting absorbance decay obtained from buffer without cell lysates by using NADPH extinction coefficient of 6220/M/cm and by normalizing to total protein. Topic. Jurkat cells were plated in a 12-well dish at a density of 8 x 10 5 cells in 1 mL media. ** Some compatible filter sets are available for. 3 Pages. USD ($) USD - United States Dollar. FilterMax F3 & F5 Microplate Readers. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). 012 fmol 0. Protein contamination can be measured at 280 nm. Fluorescence was measured using a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA, USA). 9. 0´ 104 8. 7°C (gradient heating design). SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. This assay measures cellular metabolic activity as an indicator of cell viability and proliferation. BMG Labtech - THERMOstar. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Multi-Mode Detection Platform Overview The FilterMax™ F3 and FilterMax™ F5 Multi-Mode Detection Platforms are computer-controlled multi-mode detectors capable of. Instrument Setup: 1. 7/4/2018 Great Multi-Mode Microplate Reader Chirag Patel Our lab studies the role of mTOR signaling in CD8 T cell differentiation and function. Cells were co-transfected with 2 μg total plasmid DNA (1760 ng empty vector or proviral plasmid DNA. 7/4/2018 Great Multi-Mode Microplate Reader Chirag Patel Our lab studies the role of mTOR signaling in CD8 T cell differentiation and function. Transfluor Technology. Fluorescence of the organic phase was measured at 515 nm of excitation and 553-nm emission using a fluorimeter (FilterMax F5-Multi-Mode Microplate Reader, Molecular Devices, CA, USA). Figure 1. Selecting your dsDNA assay kits The family of SpectraMax Quant dsDNA Assay Kits consist of three kits each designed for a specific range of DNA concentrations and optimized for use with florescenceThe SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. The values of luminescence are directly proportional to the number of viable cells. i3x, SpectraMax M4 or M5, and FilterMax F5 Multi-Mode Microplate Readers. LVF Monochromator™ : Excitation and Emission. There is no programming of protocols or wet chemistry involved –just select the mode(s) you want to test, insert the plate and go. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Molecular Devices technical support. d. 3 Pages. 4. com A. After initial incubation, the lysates were incubated with the caspase-1 substrate Ac-YVAD-pNA at 37 °C. Click Run. CloneMatrix. d. The results are expressed as cell viabilities (%) based on the control condition. Page 24: Synergy™ Ht Multi-Mode Microplate Reader Internal plate shaking is also supported. Cytokine concentration was determined by plotting the OD or median fluorescence intensity (MFI) of each sample on each cytokine concentration curve and multiplying the result by a dilution. Internal controls were. ~ Bio-Tek ELx808 Microplate Reader $2,795. FilterMax F5 Multi-Mode Microplate Reader. 5 million in 2019 and is projected to reach US$ 704. , Sunnyvale, CA, USA). After 2–4 h incubation at 37 °C, the medium was removed and 100 μl dimethyl sulfoxide (DMSO) was added to each well to dissolve the formazan. Selecting your dsDNA assay kits The family of SpectraMax Quant dsDNA Assay Kits consist of three kits each designed for a specific range of DNA concentrations and optimized for use with florescenceThe cells were washed again, and 90 µL of a substrate solution was added to the wells for 15 min. The measurement can be performed using a variety of detection technologies: the. 00 USD. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. Firefly and Renilla luciferase relative light units were measured using the FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA). A Vision for the Future With TTP LabTech. ” e. 2 Pages. 00 USD. The USB adaptor comes with 1 X CD that contains its USB driver. [The Multi-Mode Validation Plate offers additional testing of TRF, HTRF, and fluorescence polarization read modes on FilterMax™ F3/F5 and SpectraMax® i3x, iD5, and Paradigm® microplate readers. Cells were harvested for luciferase assay 48 h after transfection. Synergy HTX laboratory equipment pdf manual download. Maximum haemolysis was obtained using ultrapure distilled water, and untreated erythrocytes were used as a negative control [14]. Products : General, FilterMax F5 reader Please click on the links below to view the Microplate Reader Comparison Tables. Accurate measurements are ensured by automatic lamp calibration prior to each reading. Click on the microplate icon in the upper left corner and then click "New" to open a new data file: In the section "Plate1", click on the Settings icon. Approximately 50 µl of these. SpectraMax i3/i3x Multi-Mode Microplate Reader SoftMax Pro Software 0 500 1000 1500 2000 2500 3000 0 1´ 106 2´ 106 3´ 106 dsDNA (pg/we l) R F U 02 4 6 8 1 0 2. , Sunnyvale, CA, USA). (PMT) operates in photon counting mode, affording increased sensitivity for red dyes and a linear dynamic range over 6 orders of magnitude. Luminescence was measured on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, LLC, Sunnyvale, CA, USA). Upgradeable modules are also available including western blot, cell imaging, and fast. The following multi-step binary elution gradient, based on an water as phase A and acetonitrile as phase B, both containing 0. 4 Pages. (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. The performance characteristics of the data reduction software have not been established with any laboratory diagnostic assay. ** Some compatible filter sets are available for purchase from Life. Tristar 3 Multimode Microplate Reader. A standard curve was created by diluting the ATP standards to a range between 2 × 10 −3 and 20 μM ATP. Preconfigured SoftMax® Pro Software protocols (available at softmaxpro. 00. By category. Published Dec 16, 2020 | Updated Dec 16, 2020. 2 μM ATP standard was. Description Z-height focusing is enabled for fast top- and bottom-reads for all read modes. 561. Instrumentation and Quality Control. Other technologies such as imaging. Neurotransmitter Transporter Uptake Assay Kit. Installation Qualification (IQ) FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and. Open the catalog to page 1. SpectraMax Glo Steady-Luc Reporter Assay Kit. Related Instruments Agilent BioTek BioStack microplate stacker Automate routine microplate washing or dispensing processes with the compact BioStack microplate stacker. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. The increase in NADH, an end product in this reaction, was determined by. Guiberteau MD, in Essentials of Nuclear Medicine Imaging (Sixth Edition), 2012 Photon Transducers. Fluorescence polarization was measured at 21°C using FilterMax™ F5 Multimode Microplate Reader (Molecular Devices) with excitation and emission wavelengths of 485 nm and 535 nm, respectively. Perception (Vial Rack Bottom Label 2D Reader) Microscan. SpectraMax Paradigm Multi-Mode Microplate Detection System from Molecular Devices, LLC. We have 2 Molecular Devices FilterMax F5 manuals available for free PDF download:. Cell growth and viability were assessed using the CCK-8 proliferation assay. HTRF-compatible SpectraMax Multi-Mode Readers. Bioprocess Equipment and Technology. F5 Multi-Mode Microplate Reader. Enzymatic activity was calculated after subtracting absorbance decay obtained from buffer without cell lysates by using NADPH extinction coefficient of 6220/M/cm and by. RLUs were determined using a FilterMax F5 multi-mode microplate reader (Beckman Coulter). Sign In Upload. Supplier: Molecular Devices. Fluorescence intensity was measured with Filter Max F5 Multi-Mode Microplate Readers (Molecular Devices) at an excitation wavelength of 535 nm and an emission wavelength of 590 nm. FilterMax F5 Multi-Mode Microplate Reader Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm),. After 30 min, absorbance of the lysates at 405 nm was measured using a microplate reader (FilterMax F5 Multi-Mode microplate reader). 00 USD. ScanLater Western Blot Detection System. : 28 Sept 12 Page 2 of 20 Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . 4 Pages. The amount of the colorimetric product was monitored continuously for 120 min. FilterMax F5 Multi-Mode Microplate Readers, 96 to 384 Well or 6 to 1536 Well, Molecular Devices, LLC (CAT#:. State-of-the-art Multi-Mode Microplate Reader. Search LabX. We recommend you read this assay in dual emission mode, detecting both the emission from the Europium donor fluorophore at 615 nm, and the acceptor fluorophore (at 665 nm for APC. (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. 375% (w/v) thiobarbituric acid was added. #0200-7030) Filter 320/100 (available from Molecular Devices) Filter 616/10 (Molecular Devices cat. DHE fluorescence was recorded on a Filtermax F5 multi-mode microplate reader. M3/M4/M5/M5e Multi-Mode Microplate Readers + + Molecular Devices SpectraMax. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 3D cell proliferation assays were conducted using high concentrations to optimize conditions for cell lines in 20, 100 or• FilterMax™ F3/F5 Multi-Mode Microplate Readers Table 1. FilterMax F5 Multi-Mode Microplate Reader, Molecular Devices SpectraMax 340PC384 Microplate Reader, Molecular Devices. The amount of the colorimetric product was monitored continuously for 120 min. keeping with best practices, reader performance should still be validated and documented regularly, especially in regulated laboratories. FlexStation 3 Multi-Mode Microplate Reader. 2. Select the protocol “Concentration_pico_green_v3. KG (CAT#: STEM-LE-1659-Y)The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seAfter 2 hours, each well was transferred to a 96-well flat-bottom plate and the absorbance at 490 nm was measured on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA). 3 Pages. Consult Molecular Devices's entire F3/F5 Multi-Mode Microplate Readers catalogue on MedicalExpo. By performing Z-height optimization, a lens is adjusted to address low volumes or varied well geometry. Eight replicates of three independent biological samples for each experimental condition (n = 24) were used to validate the collected data. BMG Labtech - FLUOSTAR OMEGA. 5. Categories:. 713579). Each condition was performed in. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-The next day, precultures were diluted to an optical density of 0. How to Get Multi-Day Kinetic Data. Our newest multimode microplate reader combines simplicity with top-of-the-line performance. The following document is intended to demonstrate setup of this instrument. Click to Contact Seller. This minor release branch supports the B2100 (A109) hardware product until the product's End of Software. 425 or. 003 fmol 0. Synergy HTX and Synergy LX. , GFP expression yields from High Five cells infected with the two viruses were estimated using a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA). Plate Formats:. F5 has made an exception to this policy for BIG-IP 13. cruzi incubated for 1 h with sertraline at 2 μM. Protease activities were calculated from the slope of the linear portion of the normalized fluorescence vs. Once the FilterMax F5 has completely powered on, double-click on the Multi- Mode Analysis Software. For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. , Sunnyvale, CA, USA) for 30 min and 1 h after dye addition. 100 fmol 1536-well (8 µL) 0. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader $ 7,900. The results are expressed as cell viabilities (%) based on the control condition. SpectraMax Paradigm Multi-Mode Microplate Reader. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of. Readers ®Examples: Monochromators such as the SpectraMax i3 Multi-Mode Microplate Reader Detection Platform or filter based systems like the FilterMax™ F3 and F5 Multi-Mode Microplate Readers Luminescence Principle The total amount of light emitted is collected Plate specs White plates to reflect all the light to the detectorSpectraMax i3/i3x Multi-Mode Microplate Reader HeLa cells Cell viability assay SoftMax Pro Software Simplified homogenous experimental protocol The EarlyTox cell viability assay kits apply a no-wash, homogeneous protocol that simplifies the workflow and reduces the data variability typically encountered with multiple experimental steps. SpectraMax Paradigm Multi-Mode Safety Guide; SpectraMax Paradigm Multi-Mode Unpacking Guide; SpectraMax Paradigm Multi-Mode. 00. A microplate reader is laboratory machine used for the estimation of biochemical or. 3. A Filter Max F5 Multi-Mode Microplate Reader was used to measure absorbance at 595 nm. Page: 1/4NADPH turnover was measured on FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Francisco, USA) at 340 nm over 30 min at 37 °C. The OD 600 was plotted against time using Graphpad Prism 8. The hemolysis was calculated with the following formula:Luminescence from the converted ATP was then measured using a FilterMax F5 Multi-mode microplate reader (Molecular Devices). Read Review (1)FlexStation® 3 Multi-Mode Microplate Reader. Transfluor Technology. Biofilm quantification and scanning electron microscopy (SEM) analysis. 3 Pages. After the absorbance was measured at 450 nm on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA), the concentrations of the samples were determined by. It can be upgraded into a powerful multi-mode reader with up to seven detection modes. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . In addition, the FilterMax F5 Multi-Mode Microplate Do you want to learn how to use the FilterMax F3 and F5 Multi-Mode Microplate Readers for your research? Download the user guide and find out how to install, operate, and troubleshoot the instruments. FilterMax F5 Multi-Mode Microplate Reader The FilterMax F5 features absorbance (UV and visible), fluorescence intensity - FI (top and bottom reading), glow luminescence,. . Our SpectraTest® ABS2, FL1, LM1, and Multi-Mode Validation Packages provide automated,A delightfully easy to use multimode reader. VANTAstar™ Flexible Multi-mode Microplate Reader. Absorbance values recorded at 490 nm using a microplate reader (FilterMax F5, Multi-Mode Microplate Reader, Molecular Devices) were then analyzed with Excel software (Office, Microsoft) and GraphPad Prism 6 software (La Jolla, CA, USA) to assess statistical significance. 8”. Time-resolved Fluorescence. Thereafter, the dye liquid was removed, the formazan was solubilized with dimethyl sulfoxide (DMSO) under continuous agitation for 15 min, and the absorbance of each well was read by the means of a microplate reader (FilterMax F5 Multimode Microplate Reader; Molecular Devices) at a wavelength of 570 µm. Match case Limit results 1 per page. Create an experiment based on the protocol. Manufacturer: Molecular Devices. Users can program endpoint, kinetic, multiple wavelength, linear scan, and area scan measurement types. Intended Use Statement The Synergy H1 is a hybrid multi-mode microplate reader. F5 Multi-Mode Microplate Reader. Multi-mode microplate readers are used in different research fields for the quantification of different biological and chemical assays in a microplate. 2 Pages. BIO TEK Synergy? 2 Multi-Detection Microplate Reader with Luminescence and Dual Reagent Dispenser SPECTRA MAX 190 MICROPLATE READER (90V TO 240V) WITH CURRENT BMG FLUOSTAR OPTIMA PLATE READER Molecular Devices Filtermax F5 Multi-mode Microplate Reader BioLogic VMP3 Potentiostat Chassis w/9 VMP3-01 Boards potentiostat FilterMax F5 Multi-Mode Microplate Reader. Transfluor Technology. – ™FilterMax F5 Multi-Mode Microplate Reader (cat. 0. MetaFluor Software. ABS Test results. . 40266 Email: drugdiscoverytech@lifetech. 0´ 105 dsD NA (p g/ wel ) R F U • Gemini™ EM/XPS Fluorescence Microplate Readers A B C Optimal data qualityGeneral Laboratory Instruments. The 0. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Each measurement was repeated at least 3 times, and IC 50 values were calculated by non-linear regression in GraphPad Prism 9. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader. Plate Formats:. In addition, the iD5 reader can measure TRF and FP, and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter gene detection with syringe and Western blot detection. please click here. • FilterMax™ F3/F5 Multi-Mode Microplate Readers Table 1. If using a multi-functional reader, please be sure to check the sensitivity of the device using the MycoAlert™ Assay Control Set. Figure 2: Images of colonies of commonly used cell types raised in semi-solid culture in CloneMedia. BMG LABTECH. Installation Qualification (IQ)Plates were read at absorbance 405 nm using a FilterMax F5 multi-mode microplate reader (Molecular Devices, Sunnyvale, CA). Lonza Luminometers Instrument MycoAlert™ Assay MycoAlert™ PLUS Assay Other Bioassays* Notes Lonza Lucetta™ Luminometer + + Cuvette Tailor -made MycoAlert™ Mode Luminometers from Other SuppliersTriplicate reactions were measured every 2. Add to my manuals. CloneMatrix. With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. Chapter 1: Introduction The FilterMax™ F3 Multi-Mode Microplate Reader and the FilterMax™ F5 Multi-Mode Microplate Reader are ideal for a broad range of applications, including drug discovery, genomics, proteomics, and cell-based research. GPCRs. In addition, the iD5 reader can measure TRF and FP, and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter gene detection with syringe and Western blot detection. 3 Pages. Microplate Readers. Page 4 Operator’s Manual Intended Use Statement • The Synergy H1 is a hybrid multi-mode microplate reader. shopping cart 0 items $0. OMRF research scientist Kumar Subramani reviewed BioTek Synergy H1, stating it is a configurable multi-mode microplate reader with high sensitivity. Activation of TLR4 and TLR2 receptors. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. 00. Upgradeable modules are also available including western blot, cell imaging, and fast. There is no programming of protocols or wet chemistry involved –just select the mode(s) you want to test, insert the plate and go. This instrument has passed specific verification criteria. 97059-524EA 84727. Renilla luciferase activity was normalized to the background Firefly luciferase activity produced from the same psiCHECK-2 vector for each condition. Transfluor Technology. 380 or. PerkinElmer Multimode Plate Reader EnSpire 2300 $8,495. GenePix 4300/4400 Microarray Scanner. ] Example of document to evaluate if the reader . They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. 00 USD. FilterMax™ F3 and F5 Multimode Microplate Readers, Molecular Devices Supplier: Molecular Devices. Gently pull the plastic tab protruding from the microplate chamber door. 3 Pages. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. & FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. The monochromator optics uses a third generation quadruple grating design that allows working at any excitation or emission wavelength with a 1 nm step. Molecular Devices technical support is available by phone and e-mail. IMAP assays are based on binding of. 00. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. 8 Pages. FilterMax F3 & F5 Microplate Readers. FilterMax F3 & F5 Multi-Mode Microplate Readers: SpectraMax i3x Multi-Mode Microplate Reader: SpectraMax Paradigm Multi-Mode Microplate Detection System: Citations: Figures: Catalog Number: Inquire: FilterMax F3 / FilterMax F5: i3x: Inquire: Price: Description: The SpectraMax® i3 Multi-Mode Detection Platform comes with standard. Briefly, 2 h after MTS incubation at 37 °C, absorbance at 490 nm was recorded using a microplate reader (FilterMax F5, Multi-Mode Microplate Reader, Molecular Devices, San Jose, MO, USA). A dual-color pyrometer with a selected wavelength away from that of the laser radiation has been used to monitor the melt pool temperature. The absorbance of the samples was determined using a FilterMax F3&F5 Multi-Mode Microplate Reader (Molecular Devices). After 30 min, absorbance of the lysates at 405 nm was measured on a microplate reader (FilterMax F5 Multi-Mode microplate reader). They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. At least three independent experiments were performed for each. FilterMax F5 Multi–Mode microplate reader from Molecular Devices Multi–Mode Analysis software. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader. time plots at 30, 60, and 90 min using MATLAB® R2017b (9. FilterMax F5 and F3 Multi‐Mode Microplate ReadersUnpackingand Setup Guide 5016209 B 3 Setting Up the Instrument Setting up theinstrument includes selecting asuitablework area, installing excitation and emission filter slides, connecting thehost computer, and installing thesoftwarethat controls allactions performed by the instrument. MetaMorph NX. . 00 USD. 400. 3 Pages. Absorbance at 570 nm was recorded on a CLARIOstar multi-mode microplate reader (BMG Labtech, Germany). The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. 00. 2. Buffer without protease was used as a blank for background subtraction. MetaXpress. MS-3 Laser Bar Code Reader. Plates were read at absorbance 405 nm using a FilterMax F5 multi-mode microplate reader (Molecular Devices, Sunnyvale, CA). Compact designs make these instruments. 1 Software Molecular Devices’ FilterMax™ F5 Multi-Mode Microplate Reader was tested for compatibility with Life Technologies’ GeneBLAzer® assays. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. 00FilterMax F5 Multi-Mode Microplate Reader Includes Excitation Filter Slide #1 (Fluorescence: 485 nm, 535 nm; TRF: 370 nm; FP: 485 nm),. Turn on the power switch on the back of the instrument. c. FilterMax F3 and F5 Multi-Mode Microplate Readers; Absorbance Readers. , Sunnyvale, CA, USA) and normalized to the. Measurement of Caspase 3/7 Activity. Renilla luciferase activity was normalized to the background Firefly luciferase activity produced from the same psiCHECK-2 vector for each condition. ScanLater Western Blot Detection System. Ease of use. BioStack Neo is a dedicated stacker offering super-FilterMax™ F3 and FilterMax™ F5 Multi-Mode Microplate Reader User Guide 5008528 A September 2010 DTX Multimode Readers User Guide. Absorbance values were normalized on the control at 24 h and analyzed as being proportional to the number of viable cells. ScanLater Western Blot Detection System. BioStack 4 offers patented plate de-lidding and re-lidding for sensitive cell-based workflows. Page 137 Using the same plate as was used in step 3, pipette 50 µL/well of buffer • into wells C1–F5 and C10–F12. The user guide also covers the software features, the maintenance procedures, and the technical specifications of the readers. Click “Eject Plate Carrier. A standard curve was created by diluting the ATP standards to a range between 2 × 10 −3 and 20 μM ATP. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. PerkinElmer; Revvity; Customer Transformation Hub. FilterMax® F3 & F5 Multi-Mode Microplate Readers. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. 0. HEK-Blue-hTLR4 and HEK-Blue-hTLR2 cells were incubated with. 4. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. SpectraMax iD5. Reaction was stopped by the addition of 100 μl Stop Solution and optical density of each well was determined using a FilterMax F5 Multi-Mode microplate reader and SoftMax Pro 6. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumPage 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. ScanLater Western Blot Detection System. The colorimetric signal development was stopped after 10 minutes. 8 Pages. Multi-Mode Readers. Instrument Configuration: Assay Technology Adapta® & FlexStation 3 Multi-Mode Microplate Reader A five-mode microplate reader with integrated fluid transfer Key Features • Five-mode reader with wide range of applications • Flexible liquid transfer enables more assay conditions • User-defined pipetting simplifies assay optimization • Instrument and software validation Adapting to biochemical- and cell-based application requirements while. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . 25. 4. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers; SpectraMax QuickDrop Micro-Volume Spectrophotometer; EMax Plus Microplate. Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader + + Molecular Devices FlexStation. Instrument Configuration: Assay Technology Adapta® & F3/F5 Multi-Mode Microplate Readers. 9). 6 Pages. Click on the microplate icon in the upper left corner and then click FilterMax™ F5 Multi-Mode Microplate Reader: Supplier No. It allows the user to choose between monochromator-based optics for flexibility or filter-based optics for sensitivity.